Photoacoustic photocalorimetry and spectroscopy of Halobacterium halobium purple membranes
نویسندگان
چکیده
منابع مشابه
Isolation of Photochrome Transmembrane Protein Bacteriorhodopsin from Purple Membranes of Halobacterium Halobacterium Halobium. New Method for Isolation
This paper presents improved method for isolation of photochrome transmembraine protein bacteriorhodopsin (output 5 mg from 100 g of wet biomass) capable to transform light energy to electrochemical energy of generated protons H + and АТP. The protein was isolated from purple membranes of photo-organotrophic halobacterium Halobacterium halobium by cellular autolysis by distilled water, processi...
متن کاملDoubly forbidden second-harmonic generation from isotropic suspensions: Studies on the purple membrane of Halobacterium halobium.
In this paper we report results on second-harmonic generation ~SHG! from aqueous suspensions of the purple membrane of Halobacterium halobium. A detailed theory describing this SHG signal is supported by results for the angular distribution of the harmonic emission and validated by experimental measurements with circularly polarized pump radiation—a condition that normally precludes SHG. Hence ...
متن کاملGenetic variability in Halobacterium halobium.
Halobacterium halobium exhibits an extraordinary degree of spontaneous variability. Mutants which are defective in the formation of gas vacuoles (vac) arise at a frequency of 10(-2). Other easily detectable phenotypes, like the synthesis of bacterioruberin (Rub) or the synthesis of retinal (Ret) and bacterio-opsin (Ops), the two components which form the purple membrane (Pum) of H. halobium, ar...
متن کاملMolecular orientation of bacteriorhodopsin within the purple membrane of Halobacterium halobium.
The direction of orientation of the protein bacteriorhodopsin within the purple membrane of Halobacterium halobium has been determined by selected-area electron diffraction of membranes preferentially oriented by adsorption to polylysine. Purple membrane is known to adsorb preferentially to polylysine by its cytoplasmic surface at neutral pH and by its extracellular surface at low pH. To mainta...
متن کاملStudies of an acid-induced species of purple membrane from Halobacterium halobium.
A new spectral species of the purple membrane of Halobacterium halobium has been observed below pH 3.2. The formation of this new species is temperature-dependent and is favoured by increasing temperature up to the physiological range of the organism. The rate of formation at pH 3.0 and 22 degrees C is 7.9 x 10-3s-1. The spectral distribution and temperature-dependence of the new species sugges...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 1982
ISSN: 0006-3495
DOI: 10.1016/s0006-3495(82)84686-9